stemrna 3rd gen reprogramming kit reprogramming adult neonatal human fibroblasts Search Results


96
AMS Biotechnology stemrna 3rd gen reprogramming kit
Stemrna 3rd Gen Reprogramming Kit, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ReproCELL stemrna 3rd gen reprogramming kit
Stemrna 3rd Gen Reprogramming Kit, supplied by ReproCELL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ReproCELL stemrna 3rd generation reprogramming kit for adult and neonatal human fibroblasts
Human patient dermal <t>fibroblasts</t> reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Stemrna 3rd Generation Reprogramming Kit For Adult And Neonatal Human Fibroblasts, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ReproCELL healthy male dermal fibroblasts reprocellusa
Human patient dermal <t>fibroblasts</t> reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Healthy Male Dermal Fibroblasts Reprocellusa, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Coriell Institute for Medical Research fibroblasts gm08398
Human patient dermal <t>fibroblasts</t> reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Fibroblasts Gm08398, supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ReproCELL healthy male dermal fibroblasts
Human patient dermal <t>fibroblasts</t> reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Healthy Male Dermal Fibroblasts, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ReproCELL stemrna-nm reprogramming kit
Human patient dermal <t>fibroblasts</t> reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Stemrna Nm Reprogramming Kit, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ReproCELL hipsc-derived sensory neurons
Human patient dermal <t>fibroblasts</t> reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Hipsc Derived Sensory Neurons, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Thermo Fisher geltrex
Human patient dermal <t>fibroblasts</t> reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Geltrex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher lipofectamine rnaimax
Human patient dermal <t>fibroblasts</t> reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Lipofectamine Rnaimax, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ReproCELL virus cytotune ips 2 0 sendai reprogramming kit stemgent stemrna 3rd gen reprogramming kit
Human patient dermal <t>fibroblasts</t> reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Virus Cytotune Ips 2 0 Sendai Reprogramming Kit Stemgent Stemrna 3rd Gen Reprogramming Kit, supplied by ReproCELL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ReproCELL mrna reprogramming kit
Human patient dermal <t>fibroblasts</t> reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Mrna Reprogramming Kit, supplied by ReproCELL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human patient dermal fibroblasts reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells

Journal: Stem Cell Research & Therapy

Article Title: Induced pluripotent stem cells derived from patients carrying mitochondrial mutations exhibit altered bioenergetics and aberrant differentiation potential

doi: 10.1186/s13287-023-03546-7

Figure Lengend Snippet: Human patient dermal fibroblasts reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells

Article Snippet: Putative hiPSCs were generated using the StemRNA 3rd Generation Reprogramming Kit for Adult and Neonatal Human Fibroblasts (Reprocell).

Techniques: Transfection, Isolation